Anti-mouse primary antibodies for use on routine sections.

Large file ! May take a few seconds to load. All these antibodies have been tested in this Lab.
Antigen retrieval is with EDTA 10mM in MWO, with few exceptions (Eos MBP: trypsin)
We do not give dilution suggestions; each antibody works between 5 and 0.1µg/ml, with some exceptions.
These antibodies can be applied to frozen sections, but have not been tested.
Reactivty with other species: is indicated in the Note column: rat = R; human= H;

Important notice: while polyclonal antibodies may be unique by company and by lot number, monoclonal antibodies are not. Check the clone name, because identical clones are sold by different companies (e.g. B220 clone RA3/6B2 is sold by virtually any source). In the table is indicated as *.

Click on the image thumbnails to enlarge an image.

Antibody	clone /cat#	Source	species	reactivity	Notes
Lymphoid antigens
CD3e		Dako	rabbit	all T cells	R,H
CD3e	CD3-12 *	Novocastra	rat	all T cells	H
CD3z	6B10.2	SCB	mo IgG1	all T cells	Notes R, H
CD22	2D6	SBA	rat	all B cells
CD28	sc-1624	SCB	goat	T cells	R, H
CD30	mCD30.1 *	BD Pharmingen	hamster	activated cells
CD34	RAM34 *	BD Pharmingen	rat	precursors, endothelia	Notes
CD40	sc-1731	SCB	goat	Dendritic cells, B cells	H
CD45R / B220	RA3/6B2 *	SBA	rat	all B cells, NK
CD45RC	C455.1F	SBA	rat	all B cells, NK
CD79b / B29	HM79-12	SBA	rat	all B cells	Notes
CD138 Syndecan	281-2	BD Pharmingen	rat	plasmacells, epithelia
Pax5	24	BD Pharmingen	mo IgG1	all B cells	R, H
IgD		ICN	sheep	B cells
IgM		SBA	goat	B cells
IgA		SBA	goat	B cells
IgE		ICN	goat	B cells	Notes Ref
Ig isotypes (g1, g2a, g2b, g3)		SBA	goat	B cells	Ref
Mouse IgG1	X56 or A85-1	BD Pharmingen	rat	B cells
k, l		SBA	goat	B cells
Peanut Agglutinin	*	Sigma	lectin	germinal center B cells	Ref
anti-PNA		Vector	goat	anti lectin
TdT		Supertechs	rabbit	lymphoid precursors	H
CTLA-4	sc-1628	SCB	goat	subset of T cells	H
MAdCAM-1	MECA-367 *	SBA	rat	mucosal addressin, HEV

Hematopoietic antigens
Mac-2 (monocytes, macrophages)	M3/38	Cedarlane Labs	rat	all macrophages, epithelia	Notes
F4/80 (macrophages)	CI:A3-1	Serotec	rat	splenic macrophages	Notes
Myeloperoxidase		Dako	rabbit	myeloid cells	H
Eosinophilic Major basic protein		G. Gleich	rabbit	eosinophils	Ref
Lysozyme		Dako	rabbit	kidney	H
vWF		Dako	rabbit	megakaryocytes	H
Erythroid cells	TER-119 *	BD Pharmingen	rat	erythroid cells

Tissue antigens
Cytokeratins # 1,5-8,10,11,18	K8.13	Sigma	mo IgG2a	all epithelia	H
Basic CK 65-67, 64, 59, 58, 56, and 52 kD.	AE-3 *	Sigma	mo IgG1	all epithelia	H
Desmin	D33 *	Dako	mo IgG1	muscle cells	H
S-100		Dako	rabbit	FDC, nerves	H
Smooth muscle actin	1A4 *	Dako	mo IgG1	smooth muscle cells	H

Non-lineage specific
bcl-2	sc-7382	SCB	mo IgG1	ubiquitous, non-GC	H
bcl-6	sc-858	SCB	rabbit	germinal center B cells	Ref H
bcl-6	P1F6	Novocastra	mo IgG1	germinal center B cells	H
Blimp1	3H2E8	KL Calame (CU)	mo IgG1	plasmacells	Ref H
Cleaved Caspase-3		Cell Signaling Technology	rabbit	apoptotic cells	H
c-myc	sc-262	SCB	rabbit	proliferating cells	Notes H
Cyclin A	sc-751	SCB	rabbit	proliferating cells
Cyclin B1	sc-752	SCB	rabbit	proliferating cells	Notes H
Cyclin B1	GNS11	LabVision	mo IgG2a	proliferating cells	H
Cyclin D1	sc-753	SCB	rabbit	scattered cells	H
Cyclin D3	DCS22	LabVision	mo IgG1	proliferating cells	H
Cyclin D3	sc-182	SCB	rabbit	proliferating cells	H
IRF-4/ MUM1 / ICSAT	sc-6059	SCB	goat	plasmacells, T cells	H
Ki-67		Novocastra	rabbit	proliferating cells	Notes Ref H
Ki-67	sc-7846	SCB	goat	proliferating cells	Notes
Ki-67	MIB 5 ^Ref	Dako	mo IgG1	proliferating cells	H
Ki-67	Tec-3 ^Ref	J Gerdes , Dako	rat	proliferating cells
p27 / Kip1	57	BD Pharmingen	mo IgG1	quiescent cells	H
p53	CM5	Novocastra	rabbit	p53
Ribonucleotide reductase RRM1	AD203	InRo	mo IgG1	proliferating cells	H
RAG-1		BD Pharmingen	rabbit	cortical thymus	Ref , Notes
RAG-2		BD Pharmingen	rabbit	cortical thymus	Ref

Tags (Proteins, haptens)
BrdU	B44	BD Pharmingen	mo IgG1	proliferating cells
c-myc	9E10	SCB	mo IgG1		Notes
HA	3F10	Roche	rat
HA	16B12	Covance	mo IgG1
VSV	P5D4 *	Sigma	mo IgG1
FITC		Vector	goat		Notes
Phycoerythrin		Cortex	rabbit		Notes